Xpert directXtract Lysis Buffer

Αντιδραστήριο για απλή αλλά αποτελεσματική απομόνωση DNA από διάφορα δείγματα, όπως ολικό αίμα, ουρά και δείγματα αυτιού ποντικού, FTA-cards, παραφινωμένους ιστούς κ.α, σε 15 λεπτά. Το απομονωμένο DNA είναι κατάλληλο για PCR, μειώνοντας το κόστος και το χρόνο για απομόνωση DNA με άλλες μεθόδους

Xpert directXtract Lysis Buffer combines a thermostable protease with an optimized buffer, enabling simple but efficient extraction and conversation of DNA from a variety of samples, including whole blood, mouse tails and ear punches, FTA-cards, FFPE tissue, and many more. The extracted DNA is suitable for PCR, eliminating the need of time-consuming and costly DNA purification methods. DNA extraction is carried out in a single tube, without the need of multiple washing steps, therefore minimizing the risk of contamination. DNA is ready for PCR in only 15 minutes!

DNA that has been extracted with Xpert directXtract Lysis Buffer may be amplified with any optimized PCR system. However, GRiSP recommends Xpert Fast Hotstart DNA, which with its extension rate of 4-8 kb/min not only allows for fast PCR, but in combination with optimized buffer ensures high yield and sensitivity.

Samples:
The amount of required sample depends on the type of sample. Recommended quantities for 100 μl extractions are as follows:
Mouse Tail: 1 to 2 mm (approx 5 mg)
Mouse Ear Punch: 2 to 4 mm2 (approx 5 mg)
Animal Tissue: 3 to 30 mg
FFPE Tissue: 1 mm3 (or 2 mm2 of a 10 μm section)
Buccal Swab: 1 swab
Blood (mammalian): 2 to 8μl of fresh blood (with EDTA as anti-coagulant)
FTA or Guthrie card: 2 mm2
Hair follicles: 1 to 10 follicles

DNA extractions may be scaled up (or down), however, the ratio of sample to extraction volume should remain as indicated above. In case of buccal swabs, if desired, use larger volume per swab (up to 300μl) to increase yield.
QC: Functionally tested in PCR, using Xpert Fast Hotstart Mastermix (2X) with dye [#GE45]. Absence of endonucleases, exonucleases, and ribonucleases was confirmed by appropriate assays.
Storage: -20ºC for at least 1 year. No loss of performance is detected after 20 freeze/thaw cycles.