Xpert AmpliFi DNA Polymerase

Η πολυμεράση Xpert AmpliFi DNA είναι ένα ισχυρό ένζυμο υψηλής πιστότητας με αυξημένη δέσμευση στο DNA, που προσφέρει βελτιωμένη ενίσχυση, μικρότερους χρόνους επιμήκυνσης, υψηλότερη απόδοση και ικανότητα ενίσχυσης πιο δύσκολων και μεγαλύτερων τμημάτων. Σε συνδυασμό με το εξαιρετικά χαμηλό ποσοστό σφάλματος, αυτή η πολυμεράση είναι ιδανική για εφαρμογές όπως η υψηλής πιστότητας PCR, η κατευθυνόμενη μεταλλαξογένεση, η κλωνοποίηση, η PCR και η αλληλούχηση.

Xpert AmpliFi DNA Polymerase is an all-round and robust high-fidelity enzyme with enhanced DNA binding, resulting in improved processivity, shorter extension times, higher yield and capacity to amplify more difficult and longer targets. Combined with its extremely low error-rate, this makes Xpert AmpliFi ideal for applications such as high-fidelity PCR, site-directed mutagenesis, cloning, long-range PCR and DNA sequencing, when robustness and proof-reading are extremely important.

Xpert AmpliFi DNA polymerase has been derived from Pfu DNA polymerase for its proof-reading activity. Xpert AmpliFi has a very high accuracy resulting in fidelity that is about 100x higher than regular Taq DNA polymerase. DNA binding has been improved by protein engineering resulting in shorter extension times (2-6kb/min), making this also an ideal choice for consistent results in fast PCR amplifications.

Properties:
Amplicon size:  up to 20kb
Extension Rate: 2-6kb/min
Hotstart:           No
A-overhang:      No
Error-rate:  100x lower than Taq

Applications:
– High-fidelity PCR
– Complex targets (including GC-rich and AT-rich templates)
– Longe-Range PCR
– site-directed mutagenesis
– DNA Sequencing
– Cloning

Components:
– Kit version with dNTPs in separated buffer or
– Mastermix

Storage:
-20ºC for at least 1 year. No loss of performance is detected after 20 freeze/thaw cycles.