Xpert AmpliFi Hotstart DNA Polymerase

Η πολυμεράση Xpert AmpliFi DNA είναι ένα ισχυρό ένζυμο υψηλής πιστότητας με αυξημένη δέσμευση στο DNA, που προσφέρει βελτιωμένη ενίσχυση, μικρότερους χρόνους επιμήκυνσης, υψηλότερη απόδοση και ικανότητα ενίσχυσης πιο δύσκολων και μεγαλύτερων τμημάτων. Σε συνδυασμό με το εξαιρετικά χαμηλό ποσοστό σφάλματος και την πρωτοποριακή τεχνολογία αντιστρεπτής hotstart δράσης, αυτή η πολυμεράση είναι ιδανική για εφαρμογές όπως η υψηλής πιστότητας PCR, η κατευθυνόμενη μεταλλαξογένεση, η κλωνοποίηση, η PCR και η αλληλούχηση NGS.

Xpert AmpliFi Hotstart DNA Polymerase is an all-round and robust high-fidelity enzyme with enhanced DNA binding, resulting in improved processivity, shorter extension times, higher yield and capacity to amplify more difficult and longer targets. Combined with its extremely low error-rate and all the advantages of a new proprietary reversible hotstart technology, this makes Xpert AmpliFi Hotstart ideal for applications such as high-fidelity PCR, site-directed mutagenesis, multiplexing, long-range PCR and NGS sequencing, when robustness, specificity, sensitivity and proof-reading are extremely important.

Xpert AmpliFi Hotstart DNA polymerase has been derived from Pfu DNA polymerase for its proof-reading activity. Xpert AmpliFi has a very high accuracy resulting in fidelity that is about 100x higher than regular Taq DNA polymerase. Hotstart is achieved using a proprietary aptamer-like molecule that reversibly inhibits both 5´-3´polymerase activity and 3´-5´exonuclease activity. It prevents the formation of primer-dimers and non-specific amplification, increasing the sensitivity and specificity of the PCR reaction. Moreover, this allows preparing reaction mixtures at room temperature with pre- and post-PCR stability for up to 24h.

Properties:
Amplicon size:  up to 20kb
Extension Rate: 2-6kb/min
Hotstart:           Yes
A-overhang:      No
Error-rate:  100x lower than Taq

Applications:
– High-fidelity PCR
– Complex targets (including GC-rich and AT-rich templates)
– Longe-Range PCR
– site-directed mutagenesis
– NGS
– Multiplex

Components:
– Kit version with dNTPs in separated buffer or
– Mastermix

Storage:
-20ºC for at least 1 year. No loss of performance is detected after 20 freeze/thaw cycles.

GE37.0100Xpert ampliFi Hotstart DNA Polymerase (dNTPs included)100UGE47.0100Xpert ampliFi Hotstart 2X Mastermix1.25mLGE47.5100Xpert ampliFi Hotstart 2X Mastermix5x 1.25mL