Xpert One-Step Fast Probe

Χρησιμοποιώντας ειδικούς εκκινητές, το Xpert One-Step Fast Probe κιτ επιτρέπει τη σύνθεση cDNA και την επακόλουθη qPCR αντίδραση σε ένα φιαλίδιο, μειώνοντας σημαντικά τον κίνδυνο επιμόλυνσης και τον χρόνο χειρισμού. Αποτελείται από ένα μείγμα RTase, Fast qPCR Mastermix (Probe) και ξεχωριστά φιαλίδια ROX χρωστικής.

Using gene-specific primers (GSP), Xpert One-Step Fast Probe allows for first-strand cDNA synthesis and subsequent qPCR in a single-tube reaction procedure, decreasing contamination risk and reducing hands-on time considerably. Xpert One-Step Fast Probe consists of a RTase mix, a Fast qPCR Mastermix (Probe), and separated vial(s) of ROX

The RTase mix comprises a genetically modified thermostable MMLV reverse transcriptase with improved synthesis efficiency, lacking any RNase H activity, and an advanced RNase inhibitor to impede RNA degradation. The Fast qPCR Mastermix (Probe) consists of the combination of a highly efficient enzyme with a novel low inhibitory technology, which prevents the formation of unwanted primer-dimers and non-specific products, thus allowing for extremely high sensitivity and specificity. It can be used in qPCR based on a wide range of probe-based technologies, including Taqman^®, Molecular Beacons^® and Scorpion probes^®.

Xpert One-Step Fast Probe can be used to quantify virtually any RNA target, whether using mRNA, viral RNA or total RNA as template, including extremely low-copy number targets, with minimal effort and optimization.

Applications:
One-Step RT-qPCR,
bsolute quantification,
Gene expression analysis,
Low-copy number target gene detection.

Properties:
Excellent signal with low PCR inhibition
Early Ct values – Rapid extension rate
Allows for standard and fast cycling

Samples:                    
total RNA: 1pg – 1µg, mRNA: >0.01pg
Storage:
-20ºC for at least 1 year. Repeated thaw/freeze cycles (up to 25) have no negative impact on performance.