Total RNA Kit – Plant

Αποτελεσματική και γρήγορη μέθοδος για την απομόνωση ή/και τη συγκέντρωση ολικού RNA υψηλής ποιότητας (συμπεριλαμβανομένων των mRNA, tRNA και rRNA) από φυτικούς ιστούς και κύτταρα.

The GRS Total RNA Kit – Plant – provides an efficient and fast method for the purification and or concentration of high quality total RNA (including mRNA, tRNA and rRNA) from plant tissue and cells. Optional DNAse treatment can be included in the protocol to remove undesired DNA residue. Eluted purified RNA is suitable for RT-PCR, One-step qRT-PCR, Northern Blotting, mRNA selection, cDNA
synthesis, and primer extension.

Principle
Samples are ground in liquid nitrogen and subsequently filtered to remove debris. The buffer system is optimized to allow selective binding of RNA to the glass fiber matrix of the spin column (1). Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified RNA is subsequently eluted with RNAse-Free Water. The entire procedure can be completed within 30 minutes with typical RNA yields of 5-30μg without the use of phenol extraction.

Sample : up to 100mg of fresh plant tissue, up to 25 mg of dry plant tissue
Expected Yield : 5-30μg total RNA from young leaf samples
Format : spin column (certified DNAse/RNAse-free)
Operation Time : within 30 minutes
Elution Volume : 50μl