Lambda/HindIII

Lambda/HindIII DNA marker, consisting of 8 fragments ranging from 125bp to 23,130bp, supplied om a loading dye and ready-to-use.

Κατηγορία:

Lambda/HindIII is a classic DNA marker, consisting of 8 fragments that have been obtained from digesting λ DNA with the HindIII restriction enzyme. GRiSP’s Lambda/HindIII is supplied in a loading dye and is ready-to-use. It is suitable for sizing linear double-stranded DNA over a wide range of sizes.

Contents(bp)
125 | 564 | 2027 | 2322 | 4361 | 6557 | 9416 | 23130

due to complementary cohesive ends (sticky ends), some fragments may cause formation of additional bands, e.g. the 23130 bp fragment joining to the 4361 bp band to form a 27491 bp band. It is therefore recommended to separate fragments by heating at 65ºC for 3-5 minutes prior to loading.

Recommended Loading
2-5 μl/lane

Concentration
(Mixed) 40ng/μl

Storage
At least three months at room temperature, for long-time preservation, please store at -20ºC.

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