DNA Loading Buffer Blue (6X)
DNA loading Buffer Blue (6x) is a pre-mixed Loading Buffer for Agarose and Polyacrylamide gel electrophoresis, containing Bromophenol Blue as tracking dye.
GRS DNA Loading Buffer Blue (6X) is a pre-mixed Loading Buffer for Agarose and Polyacrylamide gel electrophoresis, containing Bromophenol Blue as tracking dye. EDTA is included to chelate Magnesium (up to 10 mM), stopping enzymatic reactions.
Contents
Solution of 10mM Tris-HCl pH 7,6; 60mM EDTA; 0,03% Bromophenol Blue; 60% Glycerol.
Applications
Preparation of DNA samples and markers for loading onto agarose and polyacrylamide gels. This Loading Buffer is a 6X concentrate: add 1μl of GRS DNA Loading Buffer Blue (6x) per 5 μl of DNA sample.
Dye Migration
Migration of the tracking dyes depend on the type of gel, concentration of the gel and the electrophoresis buffer. For example, on a 1% agarose gel the tracking dyes co-migrate with DNA fragments of the following sizes:
Bromophenol Blue
TAE: ~ 500bp
TBE: ~ 400bp
VOLTage Fast: ~ 125bp
Specifications
Free of DNases and RNases.
Quality Control
Absence of endodeoxyribonucleases, exodeoxyribonucleases, and ribonucleases was confirmed by appropriate assays.
Storage
Store at room temperature or +4ºC for up to at least 12 months. For longer storage, store at -20ºC.
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Xpert Green DNA Stain is a new and safe alternative to ethidium bromide (EtBr) for the visualization of DNA (double-stranded and single-stranded DNA) and RNA in agarose and polyacrylamide gels. The dye has been developed for in-gel staining and is compatible with both UV and Blue LED transilluminators.
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