TripleXtractor directRNA Kit

Το κιτ TripleXtractor directRNA συνδυάζει την ισχυρή ικανότητα λύσης του μονοφασικού διαλύματος φαινόλης/γουανινής «TripleXtractor» με ένα σύστημα στηλών για απλή και γρήγορη (εντός 15 λεπτών) απομόνωση υπερκαθαρού RNA χωρίς την ανάγκη διαχωρισμού φάσεων με χλωροφορμίου και ισοπροπανόλης.

The TripleXtractor directRNA kit combines the strong lysis capability of the monophasic phenol/guanidine thiocyanate solution “TripleXtractor Direct” with a spin column system for convenient, rapid and cost effective purification of ultrapure RNA without the need of chloroform phase separation and isopropanol precipitation.

Principle
Following a one-step homogenization/lysis step with TripleXtractor Direct, in which cells are disrupted and cellular components are dissolved efficiently, while maintaining RNA integrity, the sample is passed through a RNA binding spin column. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. Optional DNAse treatment can be included
in the protocol. The purified RNA is subsequently eluted by RNAse-free water. The entire procedure can be completed in approximately 15 minutes with a typical A260/A280>1,9 and A260/A230 >1,9. The purified total RNA is free of DNA and proteins and can be used for all common downstream applications such as cDNA Library construction, Nothern Blotting, RT-PCR, in vitro translation, Nuclease Protection Assays, etc.

Sample : Up to 200 μl of body fluids (whole blood, buffy coat, serum, etc) up to 5×106 cultured animal yeast or plant cells, 50-100mg of tissue, up to 1×109 bacterial cells.
Expected Yield : total RNA (e.g. up to 3 μg from 200 μl of whole human blood, up to 20 μg from 1×109 cells of Escherichia coli)
Format : spin column (certified DNAse/RNAse-free)
Operation Time: within 15 minutes
Elution Volume: 20-50 μl