HighFidelity DNA Πολυμεράση

Η πολυμεράση Xpert HighFidelity DNA polymerase είναι ένα ισχυρό ένζυμο με εξαιρετικά χαμηλό ποσοστό σφάλματος. Συνδέεται ισχυρά στο DNA κι έτσι έχει βελτιωμένη ενίσχυση, απόδοση και ποσοστό λάθους 50 φορές χαμηλότερο από μια απλή Taq πολυμεράση.

For amplifications with extremely low error-rate Xpert HighFidelity DNA polymerase is a robust enzyme with enhanced DNA binding, resulting in improved processivity, yield, and extremely low error-rate (50x lower than Taq DNA polymerase)

Main application: high-fidelity amplification with enhanced speed and robustness

Xpert HighFidelity DNA polymerase is a robust enzyme with enhanced DNA binding, resulting in improved processivity, yield, and extremely low error-rate, ideal for applications such as high-fidelity PCR, site-directed mutagenesis, crude sample PCR, blunt-end cloning, among others, where robustness and proof-reading are important.
The Xpert HighFidelity DNA polymerase has an error-rate of approximately 1 error per 4.5×107 nucleotides incorporated, which is 50x lower than Taq DNA polymerase.
The velocity of Xpert HighFidelity DNA polymerase allows the use of an extension rate of up to 2kb/min, making this also an ideal choice for consistent results in fast PCR amplifications.

Performance highlights:
– extremely low error-rate (50X lower then Taq)
– robust amplification even for complex targets

Properties:
Amplicon size:  up to 10kb
Extension Rate: 2kb/min
Hotstart:            No
A-overhang:       No
Fidelity:             50x (compared to Taq)

Applications:
– High-Fidelity PCR
– blunt-end cloning
– site-directed mutagenesis.

Components:
– Kit version with dNTPs included (in the buffer)

Storage:
-20ºC for at least 1 year.